TY  - THES
N1  - Pembimbing: Anti Damayanti Hamdani., MMolBio

ID  - digilib12173
UR  - https://digilib.uin-suka.ac.id/id/eprint/12173/
A1  - NOR SETYOWATI , NIM. 08640015
Y1  - 2013/08/26/
N2  - Arrowroot (Maranta arundinacea L) is one of tuber crops in Daerah Istimewa
Yogyakarta that its commercial cultivation restricted by unavailability of high
yield varieties. The information about genetic variation besides morphology
information is needed to get accurate superior characteristic of parental plant.
Molecular genetic analysis of plants relies on high yield and high purity of DNA
as well as optimized condition of molecular reactions. This study aimed to
develop suitable protocol for DNA extraction from M. arundinacea leaf and to
optimize condition of RAPD-PCR. Modification of standard plant DNA
extraction by Doyle & Doyle (1990) consistently yielded good purity and quantity
of DNA than that of Deng et al., (1995) method. Application of Doyle & Doyle
(1990) extraction method in twenty-two garut leaf bud, representations of each
regency in DIY, and two ginger plant leaf buds yielded DNA concentration from
244.14 to 1446.46 ?g/ml with A260/A280 ranged between 1.60 to 2.00, and A260
ranged between 0.09 to 0.57. In details, 15 samples had A260/A280 value from 1.82
to 1.99, 7 sample had the value of A260/A280 from 1.71 to 1.78, and the remaining
two had A260/A280 value of 1.60 and 2.0. Optimization of PCR conditions for
RAPD analysis of Garut was still needed because the applied PCR condition had
not amplified all tested samples and the amplicons showed low polimorfism.


PB  - UIN SUNAN KALIJAGA
KW  - Keywords: DNA extraction
KW  -  garut (Maranta arundinacea L.)
KW  -  RAPD-PCR




M1  - skripsi
TI  - TANAMAN GARUT (Maranta arundinacea L.)
LOKAL DIY


AV  - restricted
ER  -