eprintid: 26640
rev_number: 15
eprint_status: archive
userid: 71
dir: disk0/00/02/66/40
datestamp: 2017-07-26 07:07:58
lastmod: 2017-07-31 07:58:06
status_changed: 2017-07-26 07:07:58
type: thesis
metadata_visibility: show
creators_name: SURYANI, NIM. 11640005
title: ISOLASI DAN IDENTIFIKASI BAKTERI ASAM LAKTAT
PENGHAMBAT ALFA GLUKOSIDASE DARI BUAH KERSEN
(Muntingia calabura L.) DAN BUAH CIPLUKAN (Physalis angulata L.)
ispublished: pub
subjects: BB
divisions: jur_bio
full_text_status: restricted
keywords: alpha-glucosidase, lactic acid bacteria, antidiabetic, Muntingia
calabura, Physalis angulata
note: Andri Frediansyah, M.Sc., Jumailatus Solihah, M.Biotech.
abstract: Alpha-glucosidase enzyme plays a role in hydrolyzing of complex carbohydrates
into simple sugars, so that one alternative treatment of diabetes is to hinder the
performance of the enzyme. In general, the use of oral diabetes medications could
cause side effects. Exploration of lactic acid bacteria from kersen fruit (Muntingia
calabura L.) and ciplukan fruit (Physalis angulata L.) is one way to get new
inhibitor agent of the enzyme alpha-glucosidase. Bacterial isolation was
performed by inoculating the samples on agar medium (pour plate). The isolates
were further purified by streak plate technique then conventionally characterized
and tested for their activity of the enzyme alpha-glucosidase inhibition. The assay
for activity of the enzyme alpha-glucosidase inhibition was performed using a
microplate reader 96-wellplate with p-Nitrophenyl-α-D-glucopyranoside as
substrate. Identification of lactic acid bacteria with 16S rDNA. The bacterial
isolation resulted 31 isolates of lactic acid bacteria. The highest alpha-glucosidase
inhibition value resulted from kersen isolates the TL 2.7 was 69.90 ± 0.02% and
from ciplukan isolates CP 7.7 was 69.74 ± 1.00%. The isolate TL 2.7 allegedly
was identified as Lactobacillus pentosus and the isolate CP 7.7 was identified as
Lactococcus lactis.
date: 2017-03-16
date_type: published
pages: 91
institution: UIN SUNAN KALIJAGA YOGYAKARTA
department: FAKULTAS SAINS DAN TEKNOLOGI
thesis_type: skripsi
thesis_name: other
citation:   SURYANI, NIM. 11640005  (2017) ISOLASI DAN IDENTIFIKASI BAKTERI ASAM LAKTAT PENGHAMBAT ALFA GLUKOSIDASE DARI BUAH KERSEN (Muntingia calabura L.) DAN BUAH CIPLUKAN (Physalis angulata L.).  Skripsi thesis, UIN SUNAN KALIJAGA YOGYAKARTA.   
document_url: https://digilib.uin-suka.ac.id/id/eprint/26640/11/11640005_BAB-I_IV-atau-V_DAFTAR-PUSTAKA.pdf
document_url: https://digilib.uin-suka.ac.id/id/eprint/26640/1/11640005_BAB-II_sampai_SEBELUM-BAB-TERAKHIR.pdf