relation: https://digilib.uin-suka.ac.id/id/eprint/46952/
title: STUDI POTENSI LIPASE DARI BAKTERI  SEBAGAI SALAH SATU KOMPONEN PENYUSUN BIODETERGEN
creator: Citra Nandya Inala, NIM. 17106030017
subject: Kimia
description: Lipase enzymes belong to a class of hydrolase enzymes that are soluble in  water and could catalyze the hydrolysis of oils and fats. Lipase could be found in  oil-contaminated soil and could be used as a biodetergent. This study aims to isolate  bacteria from the landfill soil, then apply the lipase producing bacteria as one of the  components of biodetergent. The medium used during the bacterial isolation  process was Rhodamine Olive oil Agar (ROA) and the method used when determine  the enzyme activity was copper soap colorimetry.  Lipase was purified using ion exchange chromatography method with  bentonite as the matrix and using NaCl in phosphate buffer as eluent in various  concentration. Crude extract lipases and purified lipases are continuously tested  against detergent and washing test. Based on the research results, four isolats were  obtained with enzyme activity 37.96; 31,732; 34,616; 43.008 U/ml. Lipases that  could be applied as one of content of biodetergent are crude extract lipase of S22  isolat and purified lipase from isolat S212 with eluent NaCl 0,1 M and 1 M, isolat  S211 with eluent NaCl 1 M, isolat S22 with eluent NaCl 0,5 M, and isolat S32 with  eluent NaCl 0,1 M.
date: 2021-07-16
type: Thesis
type: NonPeerReviewed
format: text
language: id
identifier: https://digilib.uin-suka.ac.id/id/eprint/46952/1/17106030017_BAB-I_IV-atau-V_DAFTAR-PUSTAKA.pdf
format: text
language: id
identifier: https://digilib.uin-suka.ac.id/id/eprint/46952/2/17106030017_BAB-II_sampai_SEBELUM-BAB-TERAKHIR.pdf
identifier:   Citra Nandya Inala, NIM. 17106030017  (2021) STUDI POTENSI LIPASE DARI BAKTERI SEBAGAI SALAH SATU KOMPONEN PENYUSUN BIODETERGEN.  Skripsi thesis, UIN SUNAN KALIJAGA YOGYAKARTA.