%A NIM. 17106030017 Citra Nandya Inala
%O Dr.rer.medic. Esti Wahyu Widowati, M.Si., M.Biotech
%T STUDI POTENSI LIPASE DARI BAKTERI
SEBAGAI SALAH SATU KOMPONEN PENYUSUN BIODETERGEN
%X Lipase enzymes belong to a class of hydrolase enzymes that are soluble in
water and could catalyze the hydrolysis of oils and fats. Lipase could be found in
oil-contaminated soil and could be used as a biodetergent. This study aims to isolate
bacteria from the landfill soil, then apply the lipase producing bacteria as one of the
components of biodetergent. The medium used during the bacterial isolation
process was Rhodamine Olive oil Agar (ROA) and the method used when determine
the enzyme activity was copper soap colorimetry.
Lipase was purified using ion exchange chromatography method with
bentonite as the matrix and using NaCl in phosphate buffer as eluent in various
concentration. Crude extract lipases and purified lipases are continuously tested
against detergent and washing test. Based on the research results, four isolats were
obtained with enzyme activity 37.96; 31,732; 34,616; 43.008 U/ml. Lipases that
could be applied as one of content of biodetergent are crude extract lipase of S22
isolat and purified lipase from isolat S212 with eluent NaCl 0,1 M and 1 M, isolat
S211 with eluent NaCl 1 M, isolat S22 with eluent NaCl 0,5 M, and isolat S32 with
eluent NaCl 0,1 M.
%K lipase, bentonite, copper soap colorimetry, biodetergent
%D 2021
%I UIN SUNAN KALIJAGA YOGYAKARTA
%L digilib46952