TY  - THES
N1  - Pembimbing: Esti Wahyu Widowati, M.Si.M.Biotech dan Nita Supriyati, Apt.M.Biotech
ID  - digilib57140
UR  - https://digilib.uin-suka.ac.id/id/eprint/57140/
A1  - Narmi Utami, NIM.: 07630031
Y1  - 2011/08/08/
N2  - Silymarin is composed of a mixture of several flavonolignan, with the most
important being silybin, silydianin and silychristin. These compounds has been
used as a therapeutic agent in many types of liver diseases, such as hepatitis and
cirrosis. With the activity of silymarin, this research is done to isolate silymarin
from the seed of Silybum marianum L. Gaertn by effective and simple metode to
obtain high levels of silymarin.
The study begins with how to deffated Silybum marianum powder with nhexane.
Pulp is divided in two and each part was macerated with methanol and
ethyl acetate. Partition of the extract are taken twice by adding n-hexane and
diethyl ether successively into both of the extracts. Purification was done by
recrystallization with two solvent systems, consisted of methanol-water (1:3).
Determination of silymarin performed by densitometry using HPTLC (High
Performance Thin-Layer Chromatography) plates as stationary phase. The mobile
phase used chloroform: acetone: formic acid (9:2:1) and Rf value for silymarin
was 0.34, 0.43, 0.57.
The results show that silymarin content of methanol extract (53.81%) is
higher than the ethyl acetate extract (38.04%). Maximum absorbance of silymarin
occured at a wavelength of 288 nm. Analysis of functional groups silymarin with
infrared spectrophotometer showed that silymarin in the different prepared
samples have functional groups such as: C = O (Carbonyl),-OH (Hydroxyl), C =
C (Aromatic), =C?H, and C-O-C (Ether).
PB  - UIN SUNAN KALIJAGA YOGYAKARTA
KW  - Silymarin
KW  -  isolasi
KW  -  Silybum marianum L. Gaertn
KW  -  FTIR
KW  - 
HPTLC-Densitometri
M1  - skripsi
TI  - IDENTIFIKASI DAN PENETAPAN KADAR SILYMARIN DARI EKSTRAK METANOL DAN EKSTRAK ETIL ASETAT BIJI Silybum marianum L. Gaertn SECARA HPTLC-DENSITOMETRI
AV  - restricted
EP  - 78
ER  -