eprintid: 74866
rev_number: 10
eprint_status: archive
userid: 12460
dir: disk0/00/07/48/66
datestamp: 2026-01-07 06:32:05
lastmod: 2026-01-07 06:32:05
status_changed: 2026-01-07 06:32:05
type: thesis
metadata_visibility: show
contact_email: muh.khabib@uin-suka.ac.id
creators_name: Dimas Rolanda Agatha, NIM.: 21106040056
title: ANALISIS KERAGAMAN GENETIK PISANG LIAR (Musa acuminata Colla) ASAL DAERAH SEMI-KERING INDONESIA DENGAN MARKER SEQUENCE-RELATED AMPLIFIED POLYMORPHISM (SRAP)
ispublished: pub
subjects: 510
divisions: jur_bio
full_text_status: restricted
keywords: Alor, Keragaman Genetik, Musa Acuminata, Nusa Tenggara Timur,
Polimorfisme, SRAP
note: Dr. Ika Nugraheni Ari Martiwi, S.Si., M.Si. dan Dr. La Ode Muhammad Muchdar Davis
abstract: The genetic diversity of wild bananas (Musa acuminata Colla) adapted to semi-arid regions has potential for breeding drought tolerance. However, studies on these genetic resources are still lacking. This study aims to reveal the level of genetic diversity of several wild banana accessions from Alor Island, East Nusa Tenggara, as a representation of semi-arid regions, and to compare them with accessions from the humid region of Cibinong, West Java, using the molecular marker Sequence-Related Amplified Polymorphism (SRAP). A total of 183 samples from 8 wild banana accessions were extracted for DNA using the CTAB method and PCR with 8 combinations of SRAP primers. The percentage of polymorphic bands from each primer combination was 100% with polymorphic information content ranging from 0.37 to 0.50. The highest Nei’s unbiased genetic diversity was found in the Alor 8.1.2 accession (0.193) and the lowest value was found in the 10H accession (0.091). The total genetic diversity (Ht) was 0.2043, within accessions (Hs) was 0.1369, among accessions (Dst) was 0,0674, with genetic differentiation (Gst) and gene flow (Nm) at 0.3300 and 1.0152, respectively. Analysis of Molecular Variance (AMOVA) showed that 72% of the diversity originated from individuals within accessions and the remaining 28% from between populations. Phylogenetic tree analysis showed that the 8 accessions were divided into 3 clusters, with the first cluster containing the Alor accessions and the second and third clusters containing the Cibinong accessions. Phylogenetic tree cluster analysis and PCoA analysis showed a tendency for accessions to cluster based on their geographical origin.
date: 2025-12-16
date_type: published
pages: 91
institution: UIN SUNAN KALIJAGA YOGYAKARTA
department: FAKULTAS SAINS DAN TEKNOLOGI
thesis_type: skripsi
thesis_name: other
citation:   Dimas Rolanda Agatha, NIM.: 21106040056  (2025) ANALISIS KERAGAMAN GENETIK PISANG LIAR (Musa acuminata Colla) ASAL DAERAH SEMI-KERING INDONESIA DENGAN MARKER SEQUENCE-RELATED AMPLIFIED POLYMORPHISM (SRAP).  Skripsi thesis, UIN SUNAN KALIJAGA YOGYAKARTA.   
document_url: https://digilib.uin-suka.ac.id/id/eprint/74866/1/21106040056_BAB-I_IV-atau-V_DAFTAR-PUSTAKA.pdf
document_url: https://digilib.uin-suka.ac.id/id/eprint/74866/2/21106040056_BAB-II_sampai_SEBELUM-BAB-TERAKHIR.pdf