eprintid: 75897
rev_number: 10
eprint_status: archive
userid: 12460
dir: disk0/00/07/58/97
datestamp: 2026-04-01 04:59:22
lastmod: 2026-04-01 04:59:22
status_changed: 2026-04-01 04:59:22
type: thesis
metadata_visibility: show
contact_email: muh.khabib@uin-suka.ac.id
creators_name: Eva Mar’atus Solihah, NIM.: 22106040061
title: SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN PADA EKSTRAK DAUN KEPEL (Stelechocarpus burahol (Blume) Hook.f. & Thomson) DENGAN PELARUT BERBEDA KEPOLARAN
ispublished: pub
subjects: 510
divisions: jur_bio
full_text_status: restricted
keywords: Aktivitas Antioksidan, Daun Kepel, Kepolaran Pelarut, LC-HRMS, Total Fenolik, Total Flavonoid
note: Shifiana Rahayu, M.Sc. dan Dr. Harto Widodo, S.P., M.Biotech
abstract: Oxidative stress triggers degenerative diseases, requiring safer exogenous antioxidants to replace synthetic ones. Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) leaves, rich in secondary metabolites, offer a potential natural antioxidant source. This study determined the effect of solvent polarities on the phytochemical profile, total phenolic-flavonoid contents, and antioxidant activity of kepel leaves. The extraction was carried out using n-hexane (non-polar), ethyl acetate (semi-polar), and ethanol (polar) solvents, yielding five types of extracts: n-hexane, ethyl acetate, ethanol, ethyl acetate II, and ethanol II. Analyses included qualitative screening, LC-HRMS, antioxidant assays (DPPH, ABTS), and total phenolic (Folin-Ciocalteu, 732 nm) and flavonoid (AlCl3, 398 nm) quantification via UV-Vis spectrophotometry. LC-HRMS showed n-hexane selectively extracted lipids. Ethyl acetate extracted terpenoids, phenolics, alkaloids, lipids, and steroids. Ethanol dominantly extracted alkaloids, quaternary ammoniums, steroids, amino acids, phenolics, terpenoids, and flavonoids. Ethanol II exhibited the highest antioxidant activity (IC50 6.64 ± 1.00 μg/mL; ABTS 2,929.34 ± 69.43 μM TE/mg), while n-hexane showed the lowest (IC50 42.15 ± 4.01 μg/mL; ABTS 576.99 ± 7.17 μM TE/mg). This aligns with the highest phenolics in ethanol II (311.65 ± 13.90 mg GAE/g), whereas single ethyl acetate yielded the highest flavonoids (141.70 ± 9.87 mg QE/g). Conclusively, solvent polarity significantly affects the phytochemical profile, phenolic-flavonoid contents, and antioxidant activity of kepel leaves.
date: 2026-03-06
date_type: published
pages: 184
institution: UIN SUNAN KALIJAGA YOGYAKARTA
department: FAKULTAS SAINS DAN TEKNOLOGI
thesis_type: skripsi
thesis_name: other
citation:   Eva Mar’atus Solihah, NIM.: 22106040061  (2026) SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN PADA EKSTRAK DAUN KEPEL (Stelechocarpus burahol (Blume) Hook.f. & Thomson) DENGAN PELARUT BERBEDA KEPOLARAN.  Skripsi thesis, UIN SUNAN KALIJAGA YOGYAKARTA.   
document_url: https://digilib.uin-suka.ac.id/id/eprint/75897/1/22106040061_BAB-I_IV-atau-V_DAFTAR-PUSTAKA.pdf
document_url: https://digilib.uin-suka.ac.id/id/eprint/75897/2/22106040061_BAB-II_sampai_SEBELUM-BAB-TERAKHIR.pdf