Institutional Repository UIN Sunan Kalijaga YogyakartaISOLASI DAN KARAKTERISASI ENZIM KITINASE DARI TANAH TIMBUNAN SAMPAH SEBAGAI ANTIFUNGI TERHADAP Fusarium oxysporumNIM.: 22106030057Ira Aisyah RifniawatiauthorLandfills are disposal sites for waste and organic residues generated from human activities, providing nutrient rich conditions that support the growth and development of various microorganisms. This study aimed to isolate chitinaseproducing bacteria from the soil of the Piyungan landfill and to determine the potential of the produced chitinase as an antifungal agent against Fusarium oxysporum. Isolation of chitinolytic bacteria from Piyungan landfill soil was carried out using nutrient agar (NA) medium supplemented with chitin as the substrate. The activity of crude chitinase extract was evaluated under various temperatures of 25, (30, 35, 40, 45, 50, and 60°C) and pH values of (3, 4, 5, 6, 7, and 8). The antifungal potential of chitinase produced by the bacterial isolates against F. oxysporum was tested in vitro using the solid dilution method. The results showed that two chitinase producing bacterial isolates, coded K33c and K31e, were successfully obtained. The chitinase activities of isolates K33c and K31e, determined using the DNS method, were 0,2632 U/mL and 0,0673 U/mL, respectively. Both isolates exhibited optimum activity at neutral pH and a temperature of 40°C. The antifungal activity of chitinase from isolates K33c and K31e against F. oxysporum showed inhibition percentages of 61,31% dan 44,43%, respectively.660 Teknologi Kimia dan Ilmu yang Berkaitan2026-07-23UIN SUNAN KALIJAGA YOGYAKARTA;FAKULTAS SAINS DAN TEKNOLOGIThesis

For work being deposited by its own author: In self-archiving this collection of files and associated bibliographic metadata, I grant Institutional Repository UIN Sunan Kalijaga Yogyakarta the right to store them and to make them permanently available publicly for free on-line. I declare that this material is my own intellectual property and I understand that Institutional Repository UIN Sunan Kalijaga Yogyakarta does not assume any responsibility if there is any breach of copyright in distributing these files or metadata. (All authors are urged to prominently assert their copyright on the title page of their work.)

For work being deposited by someone other than its author: I hereby declare that the collection of files and associated bibliographic metadata that I am archiving at Institutional Repository UIN Sunan Kalijaga Yogyakarta) is in the public domain. If this is not the case, I accept full responsibility for any breach of copyright that distributing these files or metadata may entail.

Clicking on the deposit button indicates your agreement to these terms.