Institutional Repository UIN Sunan Kalijaga YogyakartaISOLASI ENZIM KITINASE DARI TANAH TERCEMAR SEBAGAI AGEN BIOKONTROL TERHADAP JAMUR Penicillium spNIM.: 22106030053Fajar RakhaniauthorChitinase is an enzyme capable of hydrolyzing chitin into simpler compounds and has the potential to be utilized as a biocontrol agent against fungi. This study aimed to isolate and characterize chitinase-producing bacteria from contaminated soil and to determine their potential as biocontrol agents against Penicillium sp. Chitinolytic bacteria were isolated using Nutrient Agar (NA) medium containing colloidal chitin, followed by morphological and biochemical characterization. Enzyme production was carried out using medium containing colloidal chitin, while chitinase activity was determined using the DNS method by measuring absorbance at a wavelength of 540 nm. The results showed that two chitinolytic bacterial isolates, namely E105a and E105b, were obtained. Based on the enzyme activity assay at pH 7 and a temperature of 37°C, isolates E105a and E105b showed enzyme activities of 0.0371 U/mL and 0.0302 U/mL, respectively. The potential of chitinase as a biocontrol agent against Penicillium sp. was demonstrated by inhibition percentages of 47,30% for isolate E105a and 43,56% for isolate E105b.543 Kimia Analitikal, Kimia Analisis2026-08-28UIN SUNAN KALIJAGA YOGYAKARTA;FAKULTAS SAINS DAN TEKNOLOGIThesis

For work being deposited by its own author: In self-archiving this collection of files and associated bibliographic metadata, I grant Institutional Repository UIN Sunan Kalijaga Yogyakarta the right to store them and to make them permanently available publicly for free on-line. I declare that this material is my own intellectual property and I understand that Institutional Repository UIN Sunan Kalijaga Yogyakarta does not assume any responsibility if there is any breach of copyright in distributing these files or metadata. (All authors are urged to prominently assert their copyright on the title page of their work.)

For work being deposited by someone other than its author: I hereby declare that the collection of files and associated bibliographic metadata that I am archiving at Institutional Repository UIN Sunan Kalijaga Yogyakarta) is in the public domain. If this is not the case, I accept full responsibility for any breach of copyright that distributing these files or metadata may entail.

Clicking on the deposit button indicates your agreement to these terms.