ZAINATUL FUAD, NIM. 09640039 (2014) UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN AWAR-AWAR (Ficus septica Burm f) TERHADAP BAKTERI Staphylococcus aureus ATCC 29523 dan Escherichia coli ATCC 35218. Skripsi thesis, UIN SUNAN KALIJAGA.
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Text (UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN AWAR-AWAR (Ficus septica Burm f) TERHADAP BAKTERI Staphylococcus aureus ATCC 29523 dan Escherichia coli ATCC 35218 )
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Text (UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN AWAR-AWAR (Ficus septica Burm f) TERHADAP BAKTERI Staphylococcus aureus ATCC 29523 dan Escherichia coli ATCC 35218 )
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Abstract
Daun awar-awar biasanya dimanfaatkan oleh masyarakat sebagai obat tradisional untuk mengobati penyakit kulit, radang usus buntu, mengatasi bisul, obat borok, dan gigitan ular berbisa. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas ekstrak etanol daun awar-awar sebagai antibakteri terhadap Staphylococcus aureus ATCC 29523 dan Escherichia coli ATCC 35218 dengan mengetahui ada tidaknya zona hambat. Uji aktivitas antibakteri dilakukan dengan menggunakan metode difusi cakram, yaitu dengan mengamati adanya zona hambat di sekitar kertas cakram yang menunjukkan tidak adanya pertumbuhan bakteri. Hasil pengujian menunjukkan bahwa ekstrak etanol daun awar-awar mempunyai aktivitas sebagai antibakteri terhadap Staphylococus aureus ATCC 29523 dan Escherichia coli ATCC 35218 dengan kategori sedang (zona hambat >5mm). Penelitian dilanjutkan dengan menganalisis mekanisme kerja antibakteri dengan metode analisis kebocoran sel menggunakan spektrofotometer UV-Vis pada panjang gelombang 260 nm untuk mendeteksi asam nukleat dan 280 nm untuk mendeteksi protein. Hasil pengujian menunjukkan bahwa bakteri S. aureus ATCC 29523 dan E. coli ATCC 35218 yang telah dikontakkan dengan ekstrak etanol daun awar-awar tidak menunjukkan adanya kebocoran sel.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Additional Information / Supervisor: | Pembimbing : Arifah Khusnuryani, M.Si |
| Uncontrolled Keywords: | Kata kunci: Antibakteri, Daun Awar-awar, Escherichia coli ATCC 35218, Staphylococcus aureus ATCC 29523 |
| Subjects: | Biologi |
| Divisions: | Fakultas Sains dan Teknologi > Biologi (S1) |
| Depositing User: | Miftahul Ulum [IT Staff] |
| Date Deposited: | 19 Mar 2014 08:32 |
| Last Modified: | 19 Mar 2014 08:32 |
| URI: | http://digilib.uin-suka.ac.id/id/eprint/11044 |
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