FITRIA SOFIYANI , NIM. 10640029 (2014) IDENTIFIKASI ISOLAT JAMUR ENDOFIT POHON SENGON PROVENAN WAMENA BERDASARKAN ANALISIS RDNA ITS. Skripsi thesis, UNIVERSITAS ISLAM NEGERI SUNAN KALIJAGA.
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Text (IDENTIFIKASI ISOLAT JAMUR ENDOFIT POHON SENGON PROVENAN WAMENA BERDASARKAN ANALISIS RDNA ITS)
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Text (IDENTIFIKASI ISOLAT JAMUR ENDOFIT POHON SENGON PROVENAN WAMENA BERDASARKAN ANALISIS RDNA ITS)
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Abstract
Jamur endofit merupakan jamur yang terdapat pada sistem jaringan tanaman yang tidak menyebabkan gejala penyakit pada tanaman inang. Jamur endofit dapat menghasilkan senyawa antibakteri yang berpotensi sebagai agen pengendali hayati. Begitu pula dengan jamur endofit yang diisolasi dari pohon sengon provenan Wamena dapat memiliki potensi sebagai agen pengendali hayati. Sebanyak dua puluh isolat jamur endofit dengan karakter morfologi koloni yang berbeda telah diisolasi dari daun, tangkai daun, kulit batang dan ranting batang pohon. Tiga isolat diantaranya tidak disertakan dalam analisis filogenetik dikarenakan hasil sequensing yang kurang baik. Identifikasi berdasarkan sekuen rDNA ITS (ITS1-5.8S-ITS2) dan analisis filogenetik terhadap tujuh belas isolat menunjukan bahwa isolat terbagi menjadi tiga kelas taksa yaitu Sordariomycetes (75%), Dothideomycetes (20%) dan Eurotiomycetes (5%). Lima belas isolat teridentifikasi hingga tingkat genus yaitu genus Fusarium, Phomopsis (telomorf Diaporthe), Phialemonium, Colletotrichum, Lasiodiplodia, dan Penicillium. Dua isolat yang lain hanya teridentifikasi hingga tingkat ordo yaitu ordo Pleosporales. Hasil identifikasi molekular didukung oleh data morfologi masing-masing isolat. Isolat yang paling sering ditemukan yaitu jamur endofit dari genus Fusarium (40%), dan genus Phomopsis (telomorf Diaporthe) (20%).
| Item Type: | Thesis (Skripsi) |
|---|---|
| Additional Information / Supervisor: | Pembimbing : Anti Damayanti H., S.Si., M.Mol.Bio |
| Uncontrolled Keywords: | Kata kunci: Identifikasi, ITS, Jamur Endofit, Sengon |
| Subjects: | Biologi |
| Divisions: | Fakultas Sains dan Teknologi > Biologi (S1) |
| Depositing User: | Miftahul Ulum [IT Staff] |
| Date Deposited: | 01 Dec 2014 11:34 |
| Last Modified: | 01 Dec 2014 11:34 |
| URI: | http://digilib.uin-suka.ac.id/id/eprint/14737 |
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