Flow cytometric analysis of MCF-7 cell line in its treatment with leaves extract of Eugenia uniflora L

Widowati, Esti Wahyu (2011) Flow cytometric analysis of MCF-7 cell line in its treatment with leaves extract of Eugenia uniflora L. In: The Internasional Confrence on Bioscience and Biotechnology, 11-12 Oktober 2011, UIN Sunan Kalijaga-Yogyakarta.

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Abstract

The use of natural product in treatment of cancer has attracted more attention since the existing treatments have not yet given any satisfactory result. Dewandaru (Eugeniauniflora.L), one of promising medicinal plants in Indonesia, was reported to have suppressionactivity on DNA polymerase of EBV (Eipstein-Bar Virus). This study aimed to reveal thecytotoxicity of E.uniflora L leaves extract and examine more deeply whether or not thisactivity will trigger the apoptosis process of human breast cancer MCF-7. The extraction process was conducted using petroleum ether, dichloromethane andmethanol. Each extract was subjected for MTT assay for cytotoxicity analysis.Theprospective compounds were then separated using vacuum column chromatography andpreparative thin layer chromatography.MTT method was used to performed cytotoxicity testof each separated fraction on MCF-7 cells. The selected compound which show the mostpotential activity was analyze to its ICso value. MCF-7 cells whichtreated with this toxiccompound was analyze for its cell cycle using flow cytometry assay and propidium iodide staining. The results showed that the ICso of the toxic compound tested on MCF-7 cells were 10ug/mL.Flow cytometry analysis showed that this compound has capability in inducingapoptosis.Cell cycle arrest was observed in MCF-7 cells in which cell accumulation occurredin Gl phase.

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