TOXIC COMPOUNDS EXTRACTED FROMI EUGENIA UNIFLORA LAGAINST T47D CELL LINE

Widowati, Esti Wahyu (2010) TOXIC COMPOUNDS EXTRACTED FROMI EUGENIA UNIFLORA LAGAINST T47D CELL LINE. In: Proceeding of International Conference on Madicinal Plants, 21 Juli 2010, Surabaya.

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Abstract

Breast cancer is a type of cancer most commonly had by women and is the second most frequentcause of death after lung and bronchial cancer.Today, cancer treatment using natural products has attractedmore attention as the existing treatments do not provide satisfactory results. Dewandaru(Eugeniauniflora.L),also known as the Surinam Cherry,has been reported to have antioxidant activity and cansuppress DNA polymerase of EBV (Eipstein-Bar Virus); however, research on its anticancer activity has notbeen reported.Therefore,the objective of this research is to study the potential application of E. uniflora asanticancer and isolate the toxic compound on T47D cell line. Petroleum ether,dichlormethane and methanol are used to extract E. uniflora leaves.The extract wastested for its cytotoxicactivity on T47D cell line using MTT method.The toxic compounds were separatedusing vacuum column chromatography and Preparative Thin Layer Chromatography methods.Cytotoxicitytest on T47D cells was performed for extracts of each separation stage.Chemical type identification of thetoxic compounds was performed using TLC, UV and IR spectrophotometry analysis. The results showed that dichlormethane extracts of E. uniflora has cytotoxic potential,having ICsovalue of 96 μg/ml and The ICso values of the compounds was 8 μg/ml.Finally,the isolated compounds arebelieved to be of triterpenoid group.

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